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Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate



Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate



Bioresource Technology 97(10): 1161-1166



Glucoamylase [glucan 1,4-alpha-glucosidase] production has been investigated by solid-state fermentation of agro-industrial wastes generated during the processing of paddy to rice flakes (categorized as coarse, medium and fine waste), along with wheat bran and rice powder by a local soil isolate Aspergillus sp. HA-2. The highest enzyme production was obtained with wheat bran (264+or-0.64 U/gds) followed by coarse waste (211.5+or-1.44 U/gds) and medium waste (192.1+or-1.15 U/gds) using 106 spores/ml as inoculum at 28+or-2 degrees C and pH 5. A combination of wheat bran and coarse waste (1:1) gave enzyme yield compared to wheat bran alone. Media supplementation with carbon source (0.04 g/gds) as sucrose in wheat bran and glucose in coarse and medium waste increased enzyme production to 271.2+or-0.92, 220.2+or-0.75 and 208.2+or-1.99 U/gds, respectively. Organic nitrogen supplementation (yeast extract and peptone, 0.02 g/gds) showed a higher enzyme production compared to inorganic source. The optimum enzyme activity was observed at 55 degrees C and pH 5. Enzyme activity was enhanced in the presence of calcium whereas presence of EDTA gave reverse effect.

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Accession: 004516392

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PMID: 16006122

DOI: 10.1016/j.biortech.2005.05.007



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