+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene



Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene



Journal of Molecular Endocrinology 36(3): 435-448



Insulin-like growth factor-binding protein 5 (IGFBP-5) mediates involution of the mammary gland. The decrease in DNA content and mammary gland weight which accompanies involution was inhibited by prolactin (PRL) in wild-type but not transgenic mice expressing IGFBP-5. Phospho-STAT5 protein levels were significantly lower in IGFBP-5 transgenic mice during lactation suggesting that IGFBP-5 antagonises PRL signalling in the mammary epithelium. In contrast, phospho-STAT3 levels increased during involution to a similar extent in both wild-type and transgenic mice and were unaffected by PRL. PRL inhibited gene expression of matrix metalloproteinases (MMPs) 3 and 12 but not tissue plasminogen activator or plasmin in wild-type and transgenic animals. The effects of PRL on MMPs appear to be indirect since PRL failed to inhibit MMP-3, -7 or -12 expression in HC-11 cells or in a co-transfection including an activated PRL receptor, STAT5 and a MMP-3-luciferase reporter gene. PRL is a potent inhibitor, both of cell death, an effect which is suppressed by IGFBP-5, and of MMP expression, which is independent of the actions of IGFBP-5.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004523574

Download citation: RISBibTeXText

PMID: 16720715

DOI: 10.1677/jme.1.01873


Related references

Mouse TGF-alpha gene expression in normal and neoplastic mammary glands and uteri of four strains of mice with different potentials for mammary gland growth and uterine adenomyosis. Zoological Science (Tokyo) 11(6): 841-846, 1994

Morinda citrifolia (Noni) Juice Augments Mammary Gland Differentiation and Reduces Mammary Tumor Growth in Mice Expressing the Unactivated c-erbB2 Transgene. Evidence-Based Complementary and Alternative Medicine 2012: 487423, 2012

Mouse mammary tumor virus/v-Ha-ras transgene-induced mammary tumors exhibit strain-specific allelic loss on mouse chromosome 4. Proceedings Of The National Academy Of Sciences Of The United States Of America. 94(16): 8664-8669, 1997

Histology of the mammary gland. V. Effect of ACTH on the lymphoid cell counts in the mammary glands of lactat-ing rats and mice. Tohoku Jour Agric Res 9(1): 23-35, 1958

Histology of the mammary gland. V. Effect of ACTH on the lymphoid cell counts in the mammary glands of lactating mice and rats. Tohoku J. agric. Res, 9: 23-35, 1958

Studies on the mouse mammary tumor agent mta part 2 cytochemistry of amino peptidase in normal mammary gland of c 57 bl spontaneous and virus induced mammary tumors in r 111 and c 57 bl mouse strains respectively and in cell lines producing the mammary tumor virus. Acta Histochemica: 181-188, 1970

The expression of N-terminally truncated occludin causes programmed cell death in the mouse mammary gland epithelium and in a mammary epithelial cell line. Molecular Biology of the Cell 12(Supplement): 418a, 2001

A mouse mammary tumor virus-Wnt-1 transgene induces mammary gland hyperplasia and tumorigenesis in mice lacking estrogen receptor-alpha. Cancer Research 59(8): 1869-1876, 1999

Inhibition by dietary dibenzoylmethane of mammary gland proliferation, formation of DMBA-DNA adducts in mammary glands, and mammary tumorigenesis in Sencar mice. Cancer Letters 168(2): 125-132, 2001

Circadian clock and cell cycle gene expression in mouse mammary epithelial cells and in the developing mouse mammary gland. Developmental Dynamics 235(1): 263-271, 2005

Studies of mouse mammary glands. II. Cytomorphology of mammary transplants in inguinal fat pads, nipple-excised host glands, and whole mammary-gland transplants. Journal of the National Cancer Institute 39(3): 491-527, 1967

Dramatic heterogeneity of transgene expression in the mammary gland of lactating mice: a model system to study the synthetic activity of mammary epithelial cells. Journal of Histochemistry and Cytochemistry 43(5): 461-470, 1995

Cell-cell contact down-regulates expression of membrane type metalloproteinase-1 (MT1-MMP) in a mouse mammary gland epithelial cell line. Zoological Science 14(1): 95-99, 1997

Studies on the mouse mammary tumour agent (MTA). II. Cytochemistry of aminopeptidase in normal mammary gland of C57BL, spontaneous and virus-induced mammary tumours in RIII and C57BL mouse strains, respectively, and in cell lines producing the mammary tumour virus. Acta histochem, 38: 1, 181-88, 1970

Studies on the mouse mammary tumour agent (MTA). II. Cytochemistry of aminopeptidase in normal mammary gland of C57BL, spontaneous and virus-induced mammary tumours in R3 and C57BL mouse strains, respectively, and in cell lines producing the mammary tumour virus. Acta Histochemica 38(1): 181-188, 1970