Selection of RAPD primers for efficient fingerprinting in roses

Kim GiJun; Choi JeongKeun; Gi GwangYeon; Lim KiByung; Han TaeHo

Korean Journal of Horticultural Science and Technology 24(2): 253-257

2006


Accession: 004525410

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Abstract
This study was conducted to select random amplified polymorphic DNA (RAPD) primers for showing reliable polymorphism in roses. The rose genomic DNA concentration for polymerase chain reaction (PCR) was from 1 to 20 ng and the annealing temperature was from 35 to 40 degrees C. Taq polymerase concentration was efficient in 1.0 unit. Band patterns were distinguishable in 155 primers out of 191 primers and the 100 primers were reproducible. Twenty primers were selected with distinguishable and over 80% reproducible polymorphism on agarose gel. This study will support the Korean rose breeding programme and the fingerprints obtained in this study were uploaded on the internet site http://hanth.jnu.ac.kr.