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2 isometric viruses in poinsettias euphorbia pulcherrima

2 isometric viruses in poinsettias euphorbia pulcherrima

Plant Disease 64(8): 782-784

Two isometric viruses were common in commercially grown poinsettias: Fulton's poinsettia mosaic virus (PoiMV) and a new virus tentatively named poinsettia cryptic virus (PoiCV). The 2 viruses were not mechanically transmitted to common indicator plants, and they did not react with each other's antisera or antisera to 52 other isometric viruses. PoiMV sedimented as 2 components with sedimentation velocities (Srel) or .apprx. 53 and 115. After negative staining with uranyl acetate, the 2 components had average particle diameters of 25 and 31 nm. PoiCV sedimented as 1 component with Srel of about 120 and average particle diameter of 28 nm. The 2 viruses also differed in electrophoretic mobility. PoiMV was detected more reliably in infected plants with enzyme-linked immunosorbent assay or the Derrick method of immunoelectron microscopy than with the agar gel double diffusion test. The latter test usually failed with PoiCV.

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