+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15 from zea mays general properties and regulation by tryptophan



3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15 from zea mays general properties and regulation by tryptophan



Plant and Cell Physiology 21(5): 793-802



In extracts from Z. mays shoots, the presence of thiol compounds in the extraction buffer was necessary to get an active 3 deoxy-D-arabino heptulosonic acid 7-phosphate (DAHP) synthase. Its pH optimum for activity was .apprx. 7.5. Of the different cations tested, only Mn2+ was an activator. Enzyme stability was optimal in Tris-HCl buffer, pH 7.5, that contained a reducing agent, Mn2+ and a polyol. Phosphoenolpyruvate (PEP) did not stabilize the preparation significantly. The synthase exhibited high affinities for both erythrose-4-phosphate (Km: 0.24 mM) and PEP (Km:0.31 mM). Its specific activity was highest in young shoots. Corn DAHP synthase was inhibited in vitro by tryptophan. The enzyme was retarded on a tryptophan agarose affinity column, but it was removed with the bulk of protein from the same support when eluted with buffer containing tryptophan. Inhibition which was easily lost during storage at 4.degree. C was pH dependent and increased during development. Maximal inhibition, .apprx. 60% with 1 mM tryptophan, was observed in extracts from 8 day old shoots. Phenylalanine and tyrosine were not inhibitory, and no synergistic effects were observed when the aromatic amino acids were tested in combination. Isoenzymes could not be demonstrated.

(PDF emailed within 1 workday: $29.90)

Accession: 004540497

Download citation: RISBibTeXText



Related references

3-Deoxy-D-arabino heptulosonate 7-phosphate synthase from Zea mays: general properties and regulation by tryptophan. Plant and Cell Physiology 21(5): 793-802, 1980

Some kinetic properties of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Neurospora crassa. Biochemical Journal 199(3): 657-665, 1981

Some kinetic properties of the tryptophan sensitive 3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15 from neurospora crassa. Biochemical Journal 199(3): 657-666, 1981

Purification and properties of tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. Journal of Bacteriology 173(6): 1894-1901, 1991

Purification and molecular properties of the tryptophan sensitive 3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15 from neurospora crassa. Biochemical Journal 197(2): 427-436, 1981

The purification and molecular properties of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Neurospora crassa. Biochemical Journal 197(2): 427-436, 1981

Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. I. Properties and regulation of 3-deoxy-d-arabino heptulosonate 7-phosphate synthase. Archives of Microbiology 103(2): 133-140, 1975

Regulation of phospho 2 keto 3 deoxy heptonate aldolase 3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15 and anthranilate synthase ec 4.1.3.27 of pseudomonas aureofaciens. Journal of General Microbiology 121(2): 473-476, 1980

Aromatic amino acid biosynthesis in alcaligenes eutrophus h 16 part 1 properties and regulation of 3 deoxy d arabino heptulosonate 7 phosphate synthase ec 4.1.2.15. Archives of Microbiology 103(2): 133-140, 1975

The kinetics of a purified form of 3 deoxy d arabino heptulosonate 7 phosphate synthase tryptophan ec 4.1.2.15 from neurospora crassa. European Journal of Biochemistry 98(2): 431-440, 1979

The kinetics of a purified form of 3-deoxy-D-arabino heptulosonate-7-phosphate synthase (tryptophan) from Neurospora crassa. European Journal of Biochemistry 98(2): 431-440, 1979

Regulation of 3 deoxy d arabino heptulosonate 7 phosphate synthase in intrasporangium sp. Korean Journal of Applied Microbiology & Bioengineering 16(1): 38-45, 1988

Properties of 3 deoxy d arabino heptulosonate 7 phosphate synthase in intrasporangium sp. Korean Journal of Applied Microbiology & Bioengineering 16(1): 51-58, 1988

Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Plant Physiology 129(4): 1866-1871, 2002

Steady-state kinetics and inhibitor binding of 3-deoxy- D-arabino-heptulosonate-7-phosphate synthase (tryptophan sensitive) from Escherichia coli. Biochemistry (American Chemical Society) 36: 817-22, 1997