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A comparison of callus induction and plant regeneration from young embryos of different rice varieties



A comparison of callus induction and plant regeneration from young embryos of different rice varieties



National Science Council Monthly 10(2): 135-143



Callus induction and plant regeneration from young embryos of 17 rice varieties, including 7 Japonica type varieties and 10 Indica type varieties, were studied. The frequency of callus induction on MS [Murashige-Skoog] basal medium supplemented with 0.5 .times. 10-5 M 2,4-D reached about 100% in each variety. The callus was then transferred to differentiation medium consisting of MS basal medium supplemented with 1.0 .times. 10-5 M kinetin and 0.5 .times. 10-5 M naphthaleneacetic acid (NAA). The callus of Japonica type varieties exhibited poor root and shoot differentation ability whereas that of Indica type varieties showed good ability to differentiate them. A remarkable high frequency up to 80-100% of shoot regeneration was obtained from callus of 'Ai-nan-tsao 39'. The young embryos inoculated to culture medium with the scutellar side up had better results of callus formation and plant regeneration. Reducing the 2,4-D concentration to 0.05 .times. 10-5 M in the callus induction medium may increase the frequency of shoot regeneration of callus transferred subsequently to differentiation medium.

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Accession: 004561223

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