Section 5
Chapter 4,564

A comparison of solid phase extraction techniques for assay of drugs in aqueous and human plasma samples

Stewart, J.T.; Reeves, T.S.; Honigberg, I.L.

Analytical Letters 17(16): 1811-1826


ISSN/ISBN: 0003-2719
DOI: 10.1080/00032718408077184
Accession: 004563341

Six commercially available solid adsorbent materials were evaluated for their applicability as effective tools for extraction of drugs from aqueous and human plasma samples. Ten model compounds [salicylic acid, naproxen, procaine hydrochloride, imipramine hydrochloride, L-dopa, cephalexin, prednisone, methoxychlor, theophylline, ouabain] were selected as representative of the acidic, basic, amphoteric, hydrophobic and hydrophilic drug classes. Percent recovery and precision data for each model drug on each solid adsorbent material were calculated using spiked water and/or plasma samples. Octadecylsilane materials were the best overall choice for extraction of the different chemical classes of drugs from water and plasma samples, giving both highest recovery and best reproducibility data for a majority of the drugs studied. Good extractability for basic drugs could be obtained using either XAD-2, Clin-elut or cyanopropyl columns. In addition, XAD-2 gave good recoveries of amphoteric compounds and Clin-elut was good for hydrophobic drugs.

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