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A glucuronyl transferase involved in glucurono xylan synthesis in pea pisum sativum cultivar alaska epicotyls



A glucuronyl transferase involved in glucurono xylan synthesis in pea pisum sativum cultivar alaska epicotyls



Biochemical Journal 213(1): 115-122



A particulate enzyme preparation made epicotyls of 1-wk-old etiolated pea (P. sativum) seedlings incorporated glucuronic acid from UDP-D-[U-14C]glucuronic acid into a hemicellulosic polysaccharide. Optimum conditions for the incorporation include the presence of Mn2+ at 4-10 mmol/l and a pH between 5 and 6. UDP-D-xyloase at 1 mmol/l allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produces is a glucuronoxylan.

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Accession: 004576643

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