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A graded organ culture assay for the detection of muellerian inhibiting substance



A graded organ culture assay for the detection of muellerian inhibiting substance



Journal of Surgical Research 23(2): 141-148



A sensitive, reliable, semiquantitative organ culture assay was developed to detect the presence of [rat] Mullerian inhibiting substance, both in whole testicular fragments and in cell-free extracts. This in vitro assay allowed regression to occur in a manner which mimicked the in vivo regression of the male fetal Mullerian duct. Timed exposure studies demonstrated that the Mullerian duct must be exposed to the testicular fragments for at least 12 h before regression was initiated. Seventy-two h were required for the morphological changes of regression to be fully expressed. Mullerian inhibiting substance was a compound of considerable importance in sexual differentiation. This assay will allow a more systematic approach to the study of this unique fetal regressor.

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Accession: 004576817

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PMID: 886843


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