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A new technique for studying the kinetics of excision repair of dna damage in mammalian cells



A new technique for studying the kinetics of excision repair of dna damage in mammalian cells



Tsitologiya 26(4): 450-457



A technique was elaborated for analyzing the kinetics of excision repair of DNA. Inhibitors of DNA synthesis (1-.beta.-arabinofuranosylcytosine and hydroxyurea) taken together, drastically sensitize human cells to the killing effects of DNA damaging agents. This sensitization depended on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is required. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying reparation at very low doses of damaging agent and even of spontaneous damages.

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Accession: 004602288

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New method for studying the kinetics of the excision repair of DNA damages in mammalian cells. Tsitologiia 26(4): 450-457, 1984

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