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A rapid and efficient method to enrich scrapie associated fibril protein from scrapie brains of hamsters/



A rapid and efficient method to enrich scrapie associated fibril protein from scrapie brains of hamsters/



Bioscience Reports 4(2): 165-170



Scrapie hamster brains contain at least 5-10 .mu.g of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of .apprx. 26,000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF and SAF-protein copurify.

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Accession: 004615745

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