A rapid high performance liquid chromatographic method for the simultaneous measurement of 6 tri cyclic anti depressants
Kiel, J.S.; Abramson, R.K.; Morgan, S.L.; Voris, J.C.
Journal of Liquid Chromatography 6(14): 2761-2774
ISSN/ISBN: 0148-3919 Accession: 004616201
A reversed-phase high pressure liquid chromatographic procedure was developed for the quantitation of the concentration of 6 different tricyclic antidepressants [doxepin, demethyldoxepin, imipramine, desipramine, amitriptyline and nortriptyline] in the plasma of patients undergoing routine drug therapy. Plasma samples were extracted into a 97:3 hexane:isoamyl alcohol solution and then extracted back into dilute acid. A Supelcosil C-8 column with 5 .mu.m packing was used in combination with an acetonitrile/phosphate buffer/diethylamine mobile phase. At an optimized mobile phase pH of 7.22, baseline separation of all 6 tricyclic antidepressants plus the internal standard was achieved within 8 min. UV detection at 254 nm resulted in limits of detection of 2.5 .mu.g/L for each drug. The potential interferences from 13 different benzodiazepines and neuroleptics was investigated. Five of the 13 parent drugs [haloperidol, thiothixene, fluphenazine, chlorpromazine and thioridazine] and 3 metabolites interfere with this tricyclic antidepressant assay.