+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A semiselective agar medium for isolating xanthomonas campestris pathovar translucens from wheat seeds



A semiselective agar medium for isolating xanthomonas campestris pathovar translucens from wheat seeds



Phytopathology 75(3): 260-263



A semiselective agar medium (XTS agar) was developed and tested for the isolation of X. campestris pv. translucens (hereafter referred to as X. translucens) from seeds of wheat (Triticum aestivum). XTS agar contained Difco nutrient agar, glucose, cycloheximide, gentamycin and cephalexin. More than 91% of the seed-associated saprophytic bacteria washed from seeds of 6 wheat lots and 1 barley (Hordeum vulgare) lot were inhibited on XTS agar. X. translucens was isolated on XTS agar from 15 of 19 randomly obtained commercial wheat seed lots in Idaho [USA], but form only three of the 19 lots assayed on nutrient glucose agar. Comparison of results of assaying washings from samples of seed lots with different levels of natural contamination by X. translucens onto XTS agar and sowing samples of the same seed lot in the field showed the former to be more sensitive. The frequent isolation of X. translucens from wheat seed lots suggests that seed contamination may be a factor in recent epiphytotics of black chaff in sprinkler-irrigated wheat fields in southern Idaho. Wheat seeds should be assayed for X. translucens and contaminated seed lots not be grown under sprinkler irrigation.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004623298

Download citation: RISBibTeXText


Related references

An Improved Semiselective Agar Medium for Xanthomonas campestris Pathovar translucens. Bios 61(3-4): 62-68, 1990

Comparison between two semiselective agar media for isolating xanthomonas campestris pathovar carotae from carrot seeds. Phytopathology 77(8): 1241, 1987

A semiselective medium for the detection and isolation of xanthomonas campestris pathovar vesicatoria from tomato seeds. Phytopathology 80(10): 1000-1001, 1990

A highly selective medium for isolating xanthomonas campestris pathovar campestris from crucifer seeds. Phytopathology 79(3): 372, 1989

Comparison of the isolation of xanthomonas campestris pathovar campestris from crucifer seeds in four semiselective media. Phytopathology 79(10): 1140, 1989

A semiselective medium for the isolation of xanthomonas campestris pathovar vesicatoria. Phytopathology 80(4): 435, 1990

Selective medium for xanthomonas campestris pathovar translucens. Phytopathology 72(7): 936, 1982

Improved assay media for isolating xanthomonas campestris pathovar campestris from crucifer seeds. Phytopathology 72(7): 944-945, 1982

A new semiselective medium for the isolation of Xanthomonas campestris pv. campestris from crucifer seeds. Phytopathology 81(4): 449-453, 1991

Epidemiology of seedborne xanthomonas campestris pathovar translucens in winter wheat. Phytopathology 80(10): 966, 1990

Development of a New Semiselective Medium for Isolating Xanthomonas campestris pv. manihotis from Plant Material and Soil. Phytopathology 89(7): 591-597, 1999

Comparison of methods of inoculating xanthomonas campestris pathovar translucens in wheat cultivars. Pakistan Journal of Botany 20(1): 109-112, 1988

Development of a semiselective medium for isolating Xanthomonas campestris pv. musacearum from insect vectors, infected plant material and soil. Plant Pathology 56(3): 383-390, 2007

Tolerance levels of seedborne xanthomonas campestris pathovar translucens the causal agent black chaff of wheat. Civerolo, E L , Et Al (Ed ) Current Plant Science And Biotechnology in Agriculture: Plant Pathogenic Bacteria; Sixth International Conference, College Park, Maryland, Usa, June 2-7, 1985 Xxiii+1050p Kluwer Academic Publishers Group: Dordrecht, Netherlands; Boston, Massachusetts, Usa Illus 974-975, 1987

Modified XTS agar for isolation of Xanthomonas campestris pv translucens from wheat and barley seed. Phytopathology 85(10): 1182, 1995