+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A specific 1 step method for the isolation of pancreatic elastase its use to characterize aortic elastase

A specific 1 step method for the isolation of pancreatic elastase its use to characterize aortic elastase

Connective Tissue Research 9(1): 11-18

Elastase was purified from an acetone-ether powder of porcine pancreas by a 1-step affinity chromatography procedure on IgG-Sepharose 4B. The IgG was derived from a rabbit immunized with porcine pancreatic elastase and was itself isolated by affinity chromatography on elastase immobilized on the same matrix. The column was calibrated with a known elastase preparation under standardized conditions. Direct isolation of elastase from pancreatic extracts yielded .apprx. 90 mg from 2 pancreases. The enzymatic activity and electrophoretic migration in SDS(sodium dodecyl sulfate)-polyacrylamide gel of the purified elastase were equal to those of the purest commercially available enzymes. Application of this method to aorta of young pigs suggests the presence of active elastase in the aorta.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 004629325

Download citation: RISBibTeXText

Related references

A specific oligonucleotide probe based on 5S rRNA sequences for identification of Vibrio anguillarum and Vibrio ordalii. Veterinary Microbiology 43(2-3): 167-171, 1995

Specificity and reactivity of human granulocyte elastase ec and cathepsin g ec porcine pancreatic elastase ec bovine chymotrypsin ec and trypsin ec toward inhibition with sulfonyl fluorides. Biochimica et Biophysica Acta 525(1): 171-179, 1978

Isolation and biological characterization of two lipopolysaccharides and a capsular enriched polysaccharide preparation from haemophilus pleuropneumoniae. American Journal of Veterinary Research 47(7): 1433-1441, 1986

A radioimmunoassay for TA-0910, a new metabolically stable thyrotrophin-releasing hormone analogue. Journal of Pharmaceutical and Biomedical Analysis 12(6): 795-804, 1994

Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY. Acta Crystallographica Section D Biological Crystallography. 54(1): 140-142,. 1, 1998

Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication. Biochemistry 37(35): 12005-12011, 1998

Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up. Clinical Infectious Diseases 15(3): 473-480, 1992

Reactivity of human gamma G erythrocyte autoantibodies with fetal, autologous and maternal red cells. Vox Sanguinis 17(6): 525-535, 1969

Pancreatic Ductal Adenocarcinoma Derived From an Intraductal Papillary Neoplasm With Synchronous Incidental Glucagonoma: A Case Report and Literature Review. Pancreas 48(4): E24-E26, 2019

The commercial use of azadirachtin and its integration into viable pest control programmes. Pesticide Science 54(3): 285-289, 1998

Enzymochemical regioselective oxidation of steroids without oxidoreductase. Journal of the American Chemical Society 110(10): 3291-3295, 1988

Effective iron chelation following oral administration of an isoniazid pyridoxal hydrazone. British Journal of Haematology 43(3): 443-450, 1979

Peptide substrates for determination of protease activity us patent 4748116. may 31 1988. Official Gazette of the United States Patent and Trademark Office Patents 1090(5): 2505-2506, 1988

Characterization of ovine follicles destined to form subfunctional corpora lutea. Journal of Animal Science 65(6): 1595-1601, 1987

Presence of hsp65 in bacterial extracts A possible mediator of orally-induced tolerance?. Experientia (basel). 51(8): 775-779, 1995