+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

A study of ethylene and carbon di oxide evolution from ethephon in tobacco

Pesticide Biochemistry and Physiology 9(1): 1-8
A study of ethylene and carbon di oxide evolution from ethephon in tobacco
Buffers and leaf discs of mature tobacco (Nicotiana tabacum L.) were utilized to study [14C]ethylene and 14CO2 evolution from radiolabeled ethephon, (2-chloroethyl)phosphonic acid. Metabolic fate of [14C]ethephon in leaf discs was investigated by use of TLC, high-voltage paper electrophoresis, autoradiography and liquid scintillation spectroscopy. The evolution of labeled ethylene generally increased with increasing buffer pH, buffer volume and dosage of [14C]ethephon. [14C]Ethylene was evolved, increasingly with time, from [14C]ethephon either added to the buffer or applied to leaf discs. The rate of [14C]ethylene evolution was maximum during the 1st day and leveled off on the 4th day. More than 50% of the total [14C]ethylene evolution over a 96 h period was recovered during the first 24 h after [14C]ethephon application. No 14CO2 was evolved when [14C]ethephon was degraded in the presence of buffer or leaf discs. Only ethephon itself, and no detectable metabolite thereof, was discovered in the methanolic extract of the leaf disc tissue. An insignificant amount of 14C activity (approximately 2% of the extracted 14C) was detected in the residue. By means of gas chromatography, it was confirmed that in buffers and tobacco leaf tissue ethephon breaks down to release ethylene but not CO2.

(PDF 0-2 workdays service: $29.90)

Accession: 004631963

Related references

A study of ethylene and CO2 evolution from ethephon in tobacco. Pesticide biochemistry and physiology: 9 (1) 1-8, 1978

The effect of temperature on rate of ethylene evolution from ethephon and from ethephon treated leaves of sour cherry. Journal of the American Society for Horticultural Science 103(2): 199-202, 1978

Effect of 2 4 chlorophenyl thio tri ethylamine hydro chloride and ethephon treatment on carotenoid ethylene and carbon di oxide production of rin and normal tomato fruit. Hortscienceec 2: 291, 1974

Effects of ethylene carbon di oxide and ethylene carbon di oxide mixtures on the activities of membrane containing and highly purified preparations of atpase from pea cotyledon mitochondria. Canadian Journal of Biochemistry 52(12): 1091-1096, 1974

Simultaneous gas chromatographic determination of nitrogen oxygen carbon mon oxide carbon di oxide nitrous oxide sulfur di oxide methane ethane and ethylene at the parts per million level part 1. Chromatographia 11(1): 14-18, 1978

Influence of carbon di oxide on ethylene enhanced cell separation in tobacco flower pedicels. Plant Physiology (Rockville) 61(4 SUPPL): 91, 1978

Examinations on the behavior of ethylene oxide in the fumigation of foodstuffs part 2 fumigation of wheat m with radioactively marked ethylene oxide 1 2 carbon 14 carbon. Zeitschrift Fuer Lebensmittel Untersuchung Und: 365-369, 1969

Carbon di oxide and ethylene evolution by highbush blueberry fruit. Hortscience 11(5): 515-517, 1976

Ethylene and carbon di oxide evolution in etiolated bean seedling. Archives Internationales de Physiologie et de Biochimie 84(1): 151-153, 1976

Effects of tobacco mosaic virus infection on photosynthetic carbon di oxide fixation and carbon 14 di oxide incorporation into protein in tobacco d leaves. Virology 42(1): 68-77, 1970