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A study of the activity of reverse transcriptase in the cell culture line flk infected with enzootic bovine leukosis virus

A study of the activity of reverse transcriptase in the cell culture line flk infected with enzootic bovine leukosis virus

Medycyna Weterynaryjna 44(1): 14-16

There has been presented the RT test which permits the detection of BLV in a cell culture and may serve to estimate indirectly the virus concentration in the culture fluid. The test has been used to show the influence of the kind of serum in a culture medium on a virus multiplication rate. It has been stated that O-a-S serum added to the culture medium caused twice the yield of the virus in comparison to the conventional culture containing the bovine fetal serum. It has been found that the test gives reproducible results (coefficient of variation = 29%) like the other tests; particularly these presented by Rossler et al. (13), which are based on short term cell culture.

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