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A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast i. isolation and characterization of fusant between saccharomyces cerevisiae and saccharomyces diastaticus


A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast i. isolation and characterization of fusant between saccharomyces cerevisiae and saccharomyces diastaticus



Korean Journal of Applied Microbiology and Bioengineering 14(4): 305-310



To improve the starch fermentation ability of yeast, hybrids were introduced by protoplast fusion of S. cerevisiae and S. diastaticus. The protoplasts of parental auxotrophic cells were fused in the presence of 10 mM CaCl2 and 30% of polyethyleneglycol (M.W. 4,000). The frequencies of fusant formation varied depending upon the strains used and were 3.51 .times. 10-4 to 5.04 .times. 10-4 for the regenerated protoplasts. The strains capable of extensive starch hydrolysis produce only 10% to total fusants. The 4 strains were finally selected by the results of starch fermentation and genetic stability test. The DNA content and cell volume of the fusants were greater than those of the parental strains.

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