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A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast iii. isolation and characterization of fusant between saccharomyces diastaticus and candida tropicalis


A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast iii. isolation and characterization of fusant between saccharomyces diastaticus and candida tropicalis



Korean Journal of Applied Microbiology and Bioengineering 14(5): 359-364



S. diastaticus hydrolyzed .alpha.-1,4-linkage of the starch and was known fermenting yeast strain, but poorly hydrolyzed .alpha.-1,6 linkage of the starch. To improve the starch fermentation ability of yeast, we tried that protoplast fusion between S. diastaticus and C. tropicalis and finally two strains of fusants (FPDC42, FPDC43) were obtained. C. tropicalis well hydrolysis both .alpha.-1,4 and .alpha.-1,6 linkages in the starch. The protoplast of parental auxotrophic cells were fused in the presence of 10 mM CaCl2 and 35% of polyethylene glycol (M. W. 4,000). The fusion frequency was 10-5 to 10-6. Properties of the fusants (genetic stability, assimilation of carbon sources, random spore formation, copper resistance, NaCl tolerance, DNA content, cell size and growth rate) were investigated.

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