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A temperature sensitive mutant defective in mitosis and cyto kinesis

A temperature sensitive mutant defective in mitosis and cyto kinesis

Experimental Cell Research 100(2): 297-302

A temperature-sensitive mutant, ts2, of mouse leukemic cells (L5178Y) loses its viability gradually at the nonpermissive temperature (39.degree. C) but resumes normal growth when shifted to the permissive temperature (33.degree. C). At 39.degree. C the incorporation rate of thymidine is reduced on a percellbasis, whereas that of uridine and leucine is unchanged. Autoradiographic study indicates that the fraction of cells which can synthesize DNA decreases steadily with time of incubation of 39.degree. C. Accumulation of mitotic and multinucleate cells suggests that ts2 cells are defective in mitosis and cytokinesis. Experiments using synchronized cultures demonstrate that the cells shifted up at the G2, but not at the G1 phase pass through the 1st mitotic phase normally.

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Accession: 004640122

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PMID: 939255

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