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A tight seal whole cell study of the voltage dependent gating mechanism of potassium channels of protoplasmic droplets of chara corallina



A tight seal whole cell study of the voltage dependent gating mechanism of potassium channels of protoplasmic droplets of chara corallina



Plant Physiology (Rockville) 84(2): 433-437



The biophysical properties of voltage-dependent K+-channels of protoplasmic droplets of Chara corallina Klein ex Willd., em, R.D.W. were investigated using the tight-seal whole cell method. Two potassium currents were observed in voltage-clamp mode and they can be used to explain the transient membrane potential time course observed in current-clamp mode. The K+-channels are identified by the effect of tetraethylammonium chloride which blocks both currents. A two-state, constant dipole moment model is used to fit the voltage-conductance curve. From this model the minimum equivalent gating charge involved in the gating mechanism of K+-channels of Chara can be estimated.

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Accession: 004641240

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