Activated complement c 3 in synovial fluid of patients with rheumatoid arthritis and osteo arthrosis correlations with complement c 3 complement c 4 and complement c 3 proactivator influence of storage
Hasler, F.; Stojan, B.
Zeitschrift fuer Rheumatologie 40(1): 40-43
Cleavage products of C3 [complement component 3] in synovial fluid were determined by 2-dimensional electrophoresis (Laurell). Statistically significant differences were observed in rheumatoid synovial fluid compared with the osteoarthrosis group. Storage is of crucial importance for the in vitro activation of C3. In fresh synovial fluid there was a significant positive correlation between breakdown products of C3 and C3, C4 and C3-proactivator. After storage (even in -70.degree. C) this positive correlation in the rheumatoid synovial fluid changes into a negative one. This phenomenon is not observed in synovial fluid from patients with osteoarthrosis. In the rheumatoid synovial fluid C3-activating fractions in 40S, 19S and 2.5S probably are responsible for the generation of C3 cleavage products.