Activation of the alternative pathway of complement by grain part 1 complement c 3 proactivator conversion and quantification of complement consumption by rye
Olenchock, S.A.; Mull, J.C.; Major, P.C.; Gladish, M.E.; Peach, M.J.I.i ; Pearson, D.J.; Elliott, J.A.; Mentnech, M.S.
Clinical Allergy 8(2): 123-134
ISSN/ISBN: 0009-9090 Accession: 004664304
Ground whole rye and airborne rye dust of comparable size distribution were tested for their ability to activate the complement [c] cascade via the alternative pathway. Precipitin-negative pooled normal human serum was incubated with increasing amounts of the 2 rye dusts. Electrophoresis of the resultant supernatant fluids demonstrated the conversion of the proactivator of C3 to the .gamma.-migrating activator of C3. This activation was completely prevented by pre-treating the serum with the chelator EDTA, while, pre-treatment with EGTA [ethylene glycol bis(.beta.-aminoethyl ether tetraacetic acid] allowed suboptimal arc conversion, strongly implying that C was activated via the alternative pathway. Quantification of the supernatant fluids showed dose-dependent C consumption as defined by CH100 [total hemolytic C] immunodiffusion and CH50 [hemolytic C] tube hemolytic techniques. Airborne rye dust showed a greater quantitative potential than ground whole rye for activating the alternative pathway. Airborne organic dusts may have direct action on the induction of inflammatory sequelae in the lungs of sensitized and unsensitized individuals.