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Active shoot regeneration in callus culture of kiwi fruit actinidia chinensis planch



Active shoot regeneration in callus culture of kiwi fruit actinidia chinensis planch



Kagawa Daigaku Nogakubu Gakujutsu Hokoku 39(1): 21-26



Hormonal and nutritional factors affecting the callus formation and adventitious shoot regeneration from stem segment callus of kiwi fruit were investigated. Extremely active callus proliferation and shoot regeneration were found on the medium containing 4PU, compared with 2iP, BA or kinetin. NAA or 2,4-D supplemented to the medium containing 0.1 mg/l of 4PU suppressed shoot regeneration. The presence of 2,4-D in the medium severely inhibited callus growth and shoot regeneration even after the callus was transferred onto the 2,4-D free medium containing 4PU. The callus induced by 2,4-D resumed the regenerative potential on the medium containing 4PU, after 4 weeks of culture with NAA. Prolific callus formation was achieved on MS or B5 medium, whereas the shoot regeneration occurred more actively on WPM medium, followed by B5, Lepoivre and MS. By IBA treatment, the regenerated shoots could be rooted easily under non-sterile condition and grown into planklets.

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