Affinity chromatography method for measurement of glycosylated proteins a clinical comparison with glycosylated hemoglobin
Russo, E.M.K.; Dib, S.A.; Chacra, A.R.
Arquivos Brasileiros de Endocrinologia e Metabologia 29(4): 106-110
This work describes a simple and sensitive affinity chromatography method for routine measurement of glycosylated serum proteins (GP). We used the sensitive Lowry method to measure the protein eluted from the column. The results are compared with the glycosylated hemoglobin (HbA1) values measured by ion-exchange microcolumn method. The PG method discriminates between normals (1.6 .+-. 0.4%) and type I (8.0 .+-. 3.0%) and type II (7.0 .+-. 2.7) diabetic patients. The mean HbA1 were 89% and 64% higher in type I and type II diabetic patients, respectively, whereas PG were 500% and 437% higher. Type II and type I diabetic patients had 35% and 26% of the HbA1 values, respectively, within the normal range. In contrast, no overlap in GP values between normal and diabetic patients was observed. The correlation coefficient between GP vs. HbA1 were 0.84 and 0.71, respectively, in type I and type II diabetic patients. We conclude that the glycosylated protein by affinity technique is a more sensitive indicator of diabetic control than the glycosylated hemoglobin by ion-exchange chromatography.