Affinity chromatography of vitamin b 6 dependent enzymes purification of pig heart branched chain amino acid transaminase ec 22.214.171.124
Korpela, T.K.; Saarinen, R.
Journal of Chromatography 218(2): 333-342
ISSN/ISBN: 0021-9673 Accession: 004685412
Pig-heart branched-chain amino acid transaminase (EC 126.96.36.199) was purified to near homogeneity with a yield of 27%. A prepurification was performed by heat treatment, gel chromatography and DEAE-Sepharose methods. For the final step, several affinity gels were tested and the 1 containing cycloserine coupled to CNBr [cyanogen bromide]-activated Sepharose-4B was selected. This effected an additional 5-fold purification with a yield of 60%. The present affinity results are compared with corresponding studies with other aminotransferases in an attempt to find possible universal techniques for their purification.