+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Allergens in pollen from mugwort artemisia vulgaris ii. characterization of a crude and a partly purified extract of mugwort pollen with particular emphasis on the glycoprotein allergen ag 7



Allergens in pollen from mugwort artemisia vulgaris ii. characterization of a crude and a partly purified extract of mugwort pollen with particular emphasis on the glycoprotein allergen ag 7



International Archives of Allergy and Applied Immunology 80(2): 113-121



A crude and a partly purified extract of mugwort pollen were characterized with particular emphasis on the glycoprotein allergen Ag7. Crossed immunoaffinoelectrophoresis with immobilized Con A in the intermediate gel demonstrated binding of Ag7 to this lectin, indicating that Ag7 contains terminal .alpha.-D-mannopyranosyl and/or .alpha.-D-glucopyranosyl residues. Crossed immunoaffinoelectrophoresis with free Con A in the first-dimension gel demonstrated microheterogeneity in the carbohydrate moiety of the allergen. Crossed radioimmunoelectrophoresis analysis using sera from 26 mugwort-allergic patients indicated that Ag7 is an important allergen in mugwort pollen. Heat stability experiments demonstrated the presence of at least three heat-resistant allergens, one of these being Ag7. Crossed immunoelectrofocusing indicated a pI value for Ag7 of 4.3.

(PDF emailed within 1 workday: $29.90)

Accession: 004698218

Download citation: RISBibTeXText

PMID: 3710605


Related references

Allergens in pollen from mugwort artemisia vulgaris i. partial characterization of allergen preparations from mugwort pollen with emphasis on the carbohydrate moiety. International Archives of Allergy and Applied Immunology 78(2): 206-212, 1985

Isolation and characterization of a glycoprotein allergen, Art v II, from pollen of mugwort (Artemisia vulgaris L.). Molecular Immunology 27(10): 1047-1056, 1990

Structural analysis of the glycoprotein allergen Art v II from the pollen of mugwort (Artemisia vulgaris L.). Journal of Biological Chemistry 266(4): 2660-2668, 1991

Isolation and characterization of cDNA clones coding for mugwort (Artemisia vulgaris) pollen allergens. International Archives of Allergy and Immunology 124(1/3): 77-79, 2001

Immunochemical and biological characterization of a mugwort (Artemisia vulgaris) pollen extract. Allergy 40(4): 289-294, 1985

Allergens in Pollen from Mugwort ( Artemisia vulgaris L.). International Archives of Allergy and Immunology 80(2): 113-121, 1986

Allergens in Pollen from Mugwort (Artemisia vulgaris L.). International Archives of Allergy and Immunology 78(2): 206-212, 1985

Sensitivity to mugwort pollen allergens (Artemisia vulgaris). Polski Tygodnik Lekarski 45(42-44): 861-863, 1990

Allergen analysis of mugwort pollen (Artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition. Allergie und Immunologie 34(1): 3-9, 1988

Allergen analysis of mugwort pollen artemisia vulgaris by means of crossed radioimmunoelectrophoresis crie and rast inhibition. Allergie und Immunologie 34(1): 3-9, 1988

The emission of mugwort pollen (Artemisia vulgaris L.) and its flight in the air. Aerobiologia 5(1): 55-63, 1989

IgE cross-reactivity between birch pollen, mugwort pollen and celery is due to at least three distinct cross-reacting allergens: immunoblot investigation of the birch-mugwort-celery syndrome. Clinical and Experimental Allergy 26(10): 1161-1170, 1996

Purification of the glycoprotein allergen Ag7 from mugwort pollen by concanavalin A affinity chromatography. Journal of Biotechnology 16(3-4): 305-316, 1990

IgE crossreactivity between mugwort pollen (Artemisia vulgaris) and hazelnut (Abellana nux) in sera from patients with sensitivity to both extracts. Clinical and Experimental Allergy 27(10): 1203-1211, 1997

Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain. Faseb Journal 17(1): 106-108, 2002