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Alpha galactosidase ec from sweet chestnut castanea sativa seeds

Alpha galactosidase ec from sweet chestnut castanea sativa seeds

Phytochemistry (Oxford) 20(7): 1493-1496

The major sugars of fresh seeds of C. sativa are raffinose, stachyose and sucrose. Drying seeds at 25.degree. C for 14 wk increased the ratio of raffinose:stachyose from 1.1-3.5, reduced sucrose content by .apprx. 50% and decreased total extractable .alpha.-galactosidase. The enzyme activity was resolved into 2 peaks, a high MW form I (apparent MW 215,000) and a low MW form II (apparent MW 53,000). The latter form was predominant in the extract of fresh seeds whereas the former was the main form in the 14-wk dried seeds. An increase in the amount of enzyme I was observed when a buffered extract (pH 5.5) of fresh seeds was stored at 4.degree. C. Enzymes I and II had pH otpima of 4.5 and 6, respectively. Both enzymes hydrolyzed p-nitrophenyl .alpha.-D-galactoside at a much greater rate than the natural substrates raffinose, stachyose, locust bean gum and carob gum. Enzyme I showed preference for stachyose as compared to raffinose; the opposite order was observed for enzyme II.

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Accession: 004701274

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