Alterations in cell surface phenotype of t cell and b cell chronic lymphocytic leukemia cells following in vitro differentiation by phorbol ester

Shawler, D.L.; Glassy, M.C.; Wormsley, S.B.; Royston, I.

Journal of the National Cancer Institute 72(5): 1059-1064


ISSN/ISBN: 1460-2105
Accession: 004703183

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The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) was used for the induction of in vitro differentiation in primary cultures of chronic found on these cells. Three distinct phenotypes were studied: the common phenotype (sIg+, HLA-DR+, MR+, T65+, Lyt-3-); the T cell phenotype (sIg-, HLA-DR-, MR-, T65+, Lyt-3+); and a unique phenotype (sIg-, HLA-DR+, MR+, T65+, Lyt-3-). In both the common and unique phenotypes, TPA increased the expression of T65 and HLA-DR, decreased the formation of MR, and induced cytoplasmic Ig, but it did not induce Lyt-3. In the unique phenotype, TPA also induced sIg. Both the common and unique phenotypes are derived from the same lineage, probably B cell. In the T cell phenotype, TPA increased the expression of T65 and Lyt-3, but it did not induce any B cell antigens. The T cell phenotype is probably derived from a T cell lineage distinct from the 2 B cell phenotypes.