+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Aminopyrine metabolism in primary monolayer cultures of diabetic rat hepatocytes



Aminopyrine metabolism in primary monolayer cultures of diabetic rat hepatocytes



Xenobiotica; the Fate of Foreign Compounds in Biological Systems 18(5): 551-560



1. A new support system has been used which provides long-term maintenance of rat liver parenchymal cells in monolayer cultures. The cells, maintained on collagen gel/polychlorinated vinylidene film, expressed aminopyrine metabolizing activity for up to 5 days. This culture system was utilized to study the metabolism of aminopyrine in the liver cells isolated from normal, alloxan- and streptozotocin-diabetic rats. 2. Aminopyrine was metabolized at a slower rate in both types of cultured diabetic rat hepatocytes than in cultured normal rat hepatocytes, as judged from higher levels of the unchanged drug in the culture medium. 3. The formation of the metabolites 4-monoaminoantipyrine, 4-acetylaminoantipyrine and 4-formylaminoantipyrine decreased in the cultured diabetic rat hepatocytes, while that of 4-aminoantipyrine was at the same levels as controls. In contrast, 3-hydroxymethyl-2-methyl-4-dimethylamino-1-phenyl-3-pyrazolin-5-on e (AM-CH2OH) formation in the cultured diabetic rat hepatocytes increased over control value. These findings agree with in vivo results which have been reported by the authors. 4. The increase in AM-CH2OH was prevented by insulin in a dose-dependent manner. However, insulin did not affect the formation of other metabolites. These findings indicate that the amount of cytochrome P-450 isozyme involved in the oxidation of 3-methyl group may be regulated by insulin. 5. The present results, indicate that this primary culture system is a useful tool for the study of drug metabolism in diabetes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004711219

Download citation: RISBibTeXText

PMID: 3041683

DOI: 10.3109/00498258809041692


Related references

Xenobiotic metabolism and toxicity in primary monolayer cultures of hepatocytes. Niph Annals 8(2): 49-63, 1985

Phenolsulfonphthalein (phenol red) metabolism in primary monolayer cultures of adult rat hepatocytes. In Vitro 18(10): 835-842, 1982

Phenolsulfonphthalein (Phenol Red) Metabolism in Primary Monolayer Cultures of Adult Rat Hepatocytes. In Vitro 18(10): 835-842, 1982

Suitability of primary monolayer cultures of adult rat hepatocytes for studies of cholesterol and bile acid metabolism. Journal of Biological Chemistry 260(2): 1015-1019, 1985

Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein. Biochemical Journal 247(1): 79-84, 1987

Regulation of glycogen metabolism in primary cultures of rat hepatocytes. Restoration of acute effects of insulin and glucose in cells from diabetic rats. Journal of Biological Chemistry 261(2): 785-790, 1986

Preparation of primary monolayer cultures of adult rat hepatocytes. Methods in Cell Science 7(4): 169-173, 1982

Subsurface cisterns of adult rat hepatocytes in primary monolayer cultures. Archives d'Anatomie Microscopique et de Morphologie Experimentale 67(4): 213-223, 1978

Induction of vitellogenin in primary monolayer cultures of cockerel hepatocytes. Proceedings of the National Academy of Sciences of the United States of America 85(10): 3450-3454, 1988

Expression of alcohol dehydrogenase in primary monolayer cultures of rat hepatocytes. Biochemical and Biophysical Research Communications 128(1): 12-17, 1985

Regulation of glycogen metabolism in primary cultures of rat hepatocytes. Restoration of acute effects of glucose in cells from diabetic rats involves protein synthesis. Journal of Biological Chemistry 262(9): 4000-4006, 1987

Induction of cytochrome P-450-dependent hydroxylases in primary monolayer cultures of rat hepatocytes. Biochimica et Biophysica Acta - General Subjects 540(3): 402-407, 1978

Characterization of spontaneous cell death in monolayer cultures of primary hepatocytes. Archives of Toxicology 85(12): 1589-1596, 2011

Down regulation of insulin receptors in primary cultures of adult rat hepatocytes in monolayer. Endocrinology 103(2): 548-553, 1978

Primary induction of vitellogenin synthesis in monolayer cultures of amphibian hepatocytes. Journal of Cell Biology 84(2): 468-475, 1980