Amphibian sympathetic ganglia in tissue culture
Hill, C.E.; Burnstock, G.
Cell and Tissue Research 162(2): 209-233
A culture medium was developed for amphibian sympathetic nervous tissue, but it is suggested that the ionic values should be adjusted to correspond to the concentrations of salts in the plasma of particular species. The morphology, monoamine fluorescence, growth and differentiation of sympathetic ganglia of the frog Limnodynastes dumerili were studied in culture. Two types of neuron were distinguished largely according to size, namely small, 18 .times. 20 .mu.m and large, 38 .times. 2 .mu.m. The possibility that these represent 1 type at different stages in development or represent functionally distinct neurons was discussed. The sympathetic neurons were extremely sensitive to nerve growth factor (NGF) which caused an increase in the size of the cell bodies, the number of nerve fibers regenerating, the rate of axonal growth and synthesis of catecholamines. Various other cell types appearing in the cultures were described, including chromaffin, satellite, Schwann, multipolar and epithelial cells and fibroblasts, melanocytes and macrophages. The epithelial cells showed slow contractions and changes in shape.