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An enzyme linked immunosorbent assay elisa for the detection of antibodies to nairobi sheep disease virus in comparison with an indirect immunofluorescent and hemagglutination test 2. results observed with sera of experimentally infected rabbits and sheep and with african sheep sera



An enzyme linked immunosorbent assay elisa for the detection of antibodies to nairobi sheep disease virus in comparison with an indirect immunofluorescent and hemagglutination test 2. results observed with sera of experimentally infected rabbits and sheep and with african sheep sera



Zentralblatt fuer Veterinaermedizin Reihe B 31(7): 537-549



Experiences with the application of a modified indirect hemagglutination (IHA), an improved indirect fluorescent antibody technique (IFAT) and an indirect enzyme-linked immunosorbent assay (ELISA) were described to demonstrate antibodies against NSD [Nairobi sheep disease] virus (NSDV). Sera from 3 rabbits and 3 sheep experimentally infected with NSDV as well as 427 sheep sera from Kenya and 50 from South Africa and 6 human sera were investigated. In rabbit sera antibody titers up to 1:512 could be detected in IHA, and titers of .apprx. 1:256 in IFAT were observed throughout the 45 wk of the investigations; ELISA-titers were higher in some samples. Antibodies in sera from experimentally infected sheep were found in IHA between 7 and 33 and in IFAT between 12 and 33 days p.i. [post infections] (end of the experiment). The highest IHA and IFAT titers were 1:128. ELISA titers were only slightly higher, but were detectable some days later p.i. than those of the IHA and IFAT. ELISA tests gave higher levels of antibodies in sheep sera from Kenya than did the other methods with maximal titers of 1:2560. IHA titers were up to 1:1024 and titers in IFAT were up to 1:256. The highest prevalence of positive sera was in sera from the Naivasha area (95.4% ELISA, 85.5% IHA, 83.6% IFAT). The corresponding figures for sera from Isiolo and Molo Grassland were significantly lower. These results could be related to the distribution of the tick Rhipicephalus appendiculatus. Three of the 50 sheep sera from South Africa were positive only in ELISA. These were cross-reactions with antibodies against other bunyaviruses. One of 6 human sera showed low levels of antibodies in all 3 tests. This might be the result of a nosocomial subclinical infection in the donor, who handled NSDV. Sheep sera from Sudan were tested by IFAT only; 60 of 291 sera were regarded as positive, but only low titers were found. For technical reasons it is recommended to use the IHA and ELISA for seroepidemiological surveys and the IFAT to check doubtful reactions.

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An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Nairobi sheep disease virus in comparison with an indirect immunofluorescent- and haemagglutination test. II. Results observed with sera of experimentally infected rabbits and sheep and with African sheep sera. Zentralblatt für Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B 31(7): 537-549, 1984

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