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An improved direct inter microscopic light microscopic scanning electron microscopic transmission electron microscopic correlative procedure for the examination of mammalian skeletal muscle



An improved direct inter microscopic light microscopic scanning electron microscopic transmission electron microscopic correlative procedure for the examination of mammalian skeletal muscle



Journal of Microscopy (Oxford) 120(1): 53-64



The procedure for direct intermicroscopic (LM [light microscopic] .fwdarw. SEM [scanning electron microscopic] .fwdarw. TEM [transmission electron microscopic]) correlation of [mouse] muscle was modified. The major modification was the use of cryostat sections which showed far better ultrastructural preservation than did paraffin sections. The use of an osmium-thiocarbohydrazide ligand binding procedure (OTOTO) did not noticeably improve the SEM image, but did result in a subsequent TEM image which showed increased contrast of the intracellular membranous components. Besides improving the preservation at the TEM level of examination, the techniques used to locate the same structure or area previously examined under the complementary modes of microscopy (i.e., LM and SEM), were also refined.

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Accession: 004728520

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