Analysis of cysteinyldopas, dopa, dopamine, noradrenaline and adrenaline in serum and urine using high-performance liquid chromatography and electrochemical detection

Hansson, C.; Agrup, G.; Rorsman, H.; Rosengren, A.M.; Rosengren, E.; Edholm, L.E.

Journal of Chromatography 162(1): 7-22


ISSN/ISBN: 0021-9673
PMID: 33194
Accession: 004738842

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The catecholic amino acids, dopa, 2-S- and 5-S-cysteinyldopa, and 2,5-S,S-dicysteinyldopa were determined qualitatively in serum from patients with malignant melanoma by reversed-phase high-performance liquid chromatography, using electrochemical detection. In urine the catecholamines dopamine, noradrenaline and adrenaline were also determined qualitatively, as well as the above-mentioned compounds, in a single chromatographic run. The conditions were optimized by changing the pH of the mobile phase and by the addition of methanesulfonic acid. A comparison was made between the performance of 4 commercial reversed-phase packing materials containing chemically bonded octadecyl groups, using a standard mixture of catecholic amino acids. The influence of ionic strength, pH and amount of methanesulfonic acid on retention was investigated.