Analysis of deoxy cytidine deaminase activity in herpes simplex virus infected or herpes simplex virus thymidine kinase transformed cells association with mycoplasma contamination not with virus infection
Charron, J.; Langelier, Y.
Journal of General Virology 57(1): 245-250
ISSN/ISBN: 0022-1317 Accession: 004738932
Deoxycytidine (dC) deaminase activity was previously induced in herpes simplex virus (HSV)-infected cells. HSV infection of either hamster cells naturally deficient in this enzyme activity or mouse cells containing a low level of activity never resulted in appearance or stimulation of dC deaminase; thymidine kinase (TK) was always induced. The dC deaminase activity, which differed by electrophoretic mobility from the mouse or human cell enzyme, was discovered in some cells selected for the presence of HSV TK after infection with UV-irradiated HSV. The appearance of this new enzyme apparently was not due to the presence of virus genes but rather to mycoplasma contamination.