+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Analysis of protein patterns during differentiation using 2 dimensional electrophoresis and computer multi dimensional classification

Analysis of protein patterns during differentiation using 2 dimensional electrophoresis and computer multi dimensional classification

Electrophoresis 4(1): 63-70

ISSN/ISBN: 0173-0835

DOI: 10.1002/elps.1150040109

The application of a general method of comparing and sorting the patterns of protein expression obtained from 2-dimensional (2-D) electrophoresis of a mixture of proteins was described. The method involved the use of 2 multidimensional classification programs and was applied, first, to the comparison of the protein patterns expressed at different stages during nymphal differentiation of imaginal wing discs in the insect Pieris brassicae. Comparison of these stages was based on the similarity of protein expression. The technique used allowed processing of all the information visible on a gel and took into account the variations in most of the spots labeled. Two qualitatively different stages of protein expression were found during the nymphal stage, and fine variations not revealed by any other method were shown to exist in genetic programming. The method was applied to sorting the different polypeptides expressed; this was also done on the basis of their similarity of expression. Three groups of related peptides with common regulation features and apparently common physicochemical characteristics are characterized. The complete map of the highly correlated peptides, which may constitute subunits of the same protein, was constructed by the programs, thus permitting fast rational analysis of protein expression. The clinical applications of these classification techniques are discussed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004741796

Download citation: RISBibTeXText

Related references

Effects of renovascular hypertension on myocardial protein patterns Analysis by computer assisted 2-dimensional gel electrophoresis. European Heart Journal 18(ABSTR Suppl ): 84, 1997

Effects of renovascular hypertension on myocardial protein patterns: analysis by computer-assisted two-dimensional gel electrophoresis. Electrophoresis 19(11): 2043-2050, 1998

Dilated cardiomyopathy: computer-assisted analysis of endomyocardial biopsy protein patterns by two-dimensional gel electrophoresis. European Journal of Clinical Chemistry and Clinical Biochemistry: Journal of the Forum of European Clinical Chemistry Societies 32(8): 615-624, 1994

The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns. Clinical Chemistry 27(11): 1807-1820, 1981

Characterization of native multi component protein mixtures by 1 dimensional and 2 dimensional gradient electrophoresis. Journal of Chromatography 173(2): 299-312, 1979

Protein patterns of coffee beans characterization by 1 dimensional and 2 dimensional electrophoresis. Zeitschrift fur Acker- und Pflanzenbau 151(2): 89-98, 1982

Proteome analysis of secreted proteins during osteoclast differentiation using two different methods: two-dimensional electrophoresis and isotope-coded affinity tags analysis with two-dimensional chromatography. Proteomics 3(5): 616-626, 2003

GELLAB: a computer system for two-dimensional gel electrophoresis analysis. III. Multiple two-dimensional gel analysis. Computers and Biomedical Research An International Journal 14(5): 407-446, 1981

Age related changes in protein profile of rat cerebral microvessels computer analysis of two dimensional 2d electrophoresis. Clinical Research 39(1): 87A, 1991

Gnathostoma spinigerum: analysis of protein patterns by two dimensional gel electrophoresis. Southeast Asian Journal of Tropical Medicine and Public Health 31(1): 138-140, 2000

Analysis of diabetic related changes in tear protein patterns using two-dimensional electrophoresis. IOVS Investigative Ophthalmology and Visual Science 42(4): S258, 2001

Multivariate data analysis of two-dimensional gel electrophoresis protein patterns from few samples. Journal of Proteome Research 7(3): 1288-1296, 2008

Establishment and preliminary analysis of two-dimensional electrophoresis patterns of protein in ginseng callus. Zhong Yao Cai 37(9): 1540-1545, 2014

Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing: imaging of the fluorescent protein spot patterns using a cooled charge-coupled device. Analytical Biochemistry 209(1): 57-62, 1993

The early time course of calcium-binding protein induction by 1,25-dihydroxyvitamin D3 as determined by computer analysis of two-dimensional electrophoresis gels. Journal of Biological Chemistry 259(5): 3355-3360, 1984