Analysis of proteoglycans by high-performance liquid chromatography: a rapid micromethod for the separation of proteoglycans from tissue and cell culture
Iozzo, R.V.; Marroquin, R.; Wight, T.N.
Analytical Biochemistry 126(1): 190-199
ISSN/ISBN: 0003-2697 PMID: 7181110 DOI: 10.1016/0003-2697(82)90128-2
Proteoglycans were separated by high-performance liquid chromatography (HPLC), using 2 coupled Aquapore columns containing glycerylpropylsilane groups covalently linked to large-pore (50-100 nm) silica spheres. This 2-column HPLC system was effective in separating cartilage proteoglycan aggregates and monomers [from rat chondrosarcoma, calf and dog], without altering their biochemical integrity. This system was also effective in resolving small amounts of isotopically labeled proteoglycans synthesized by cultured mammalian [human fibroblast, primate smooth muscle] cells. The small sample size, short analysis time, and high reproducibility represent improvements in the study of proteoglycans over conventional soft-gel chromatography.