Section 5
Chapter 4,762

Antibiotic production by marine pigmented bacteria ii. purification and characterization of antibiotic substance of alteromonas luteoviolacea

Sakata, T.; Sakaguchi, K.; Kakimoto, D.

Memoirs of Faculty of Fisheries Kagoshima University 35(1): 29-38


Accession: 004761452

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A marine purple bacterium, Alteromonas luteoviolacea 9K-V9 isolated from sea water of Kinko Bay, Japan produced proteinous antibiotic substances. The crude extract from the cells contained two major components, lower molecular and higher molecular ones responsible for the antibiotic and autotoxic activities. The higher molecular component may composed of the lower molecular component and other cellular materials. The lower molecular substance was purified by using DEAE-sephadex A-50 chromatography, Toyopearl HW-55 gel filtration and polyacrylamide gel electrophoresis. The purified substance was shown to be heat-labile but relatively stable to a wide range of pH and to have an approximate molecular weight of 50,000.

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