Section 5
Chapter 4,763

Antibodies to human null acute lymphoblastic leukemia cell line nall 1 1. monoclonal antibodies with specificities for hla ia like and human leukocyte common antigens

Haruta, Y.

Okayama Igakkai Zasshi 96(1-2): 137-148


ISSN/ISBN: 0030-1558
Accession: 004762648

Three monoclonal antibodies, designated H-1, H-2 and H-3, were produced by immunizing BALB/C mice with cells of an established human null-acute lymphoblastic leukemia (NALL-1) cell line and fusing spleen cells from these with mice with cells of a mouse myeloma cell line, P3-NS1/1-AG4-1. The resulting hybrid cells were initially screened for production of antibodies by complement-dependent cytotoxicity. Stable monoclonal antibody-producing cell lines were isolated by repeated cloning. These independently derived monoclonal antibodies showed complement-dependent cytotoxicities. Further characterization of the monoclonal antibodies was made by immunofluorescence. H-1 antibody reacted with all tested cells of the human nucleated cells except for HLA-lacking [Burkitt's lymphoma] Daudi and [leukemia] K562 cells and was specific for a common antigenic determinant of HLA-A, -B and -C. H-2 antibody reacted with all of the tested null-cell lines, B cell lines, B cells and monocytes. Studies on fresh leukemia and lymphoma cells revealed that tumor cells from null-acute lymphoblastic leukemia, B-chronic lymphocytic leukemia and null-non-Hodgkin's lymphoma were H-2 antibody reactive. Cells from some patients with acute myelogenous leukemia, acute monocytic leukemia, chronic myelogenous leukemia in blast crisis and adult T cell leukemia were also H-2-positive. T-acute lymphoblastic leukemia, T-non-Hodgkin's lymphoma cells and mature T-cells did not react with H-2. A cross absorption study using H-2 and anti-HLA-DR monoclonal antibody confirmed the same specificity. H-2 showed blocking activities against the stimulation in 1-way mixed lymphocyte culture. H-2 is evidently a monoclonal antibody specific for a framework determinant of human Ia-like antigens. Reactivity of H-3 antibody was different from that of H-1 and H-2. H-3 reacted with some T cells, B cells and monocytes, suggesting that the antibody is associated with human leukocyte common antigens.

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