EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Antibodies to the amino terminal portion of cartilage inducing factor a and transforming growth beta immunohistochemical localization and association with differentiating cells


Antibodies to the amino terminal portion of cartilage inducing factor a and transforming growth beta immunohistochemical localization and association with differentiating cells



Journal of Biological Chemistry 261(26): 12362-12367



ISSN/ISBN: 0021-9258

Two apparently homologous proteins, designated CIF-A and CIF-B, were previously isolated from bovine bone on the basis of their cartilage-inducing activity in culture. CIF-A has been shown to probably be identical to transforming growth factor .beta. (TGF-.beta.). To address the question of tissue localization, antibodies to CIF-A were produced using a synthetic polypeptide identical to N-terminal residues 1-30. The antibodies were immunoreactive with bovine CIF-A and human TGF-.beta., did not recognize CIF-B, and did not recognize other molecular weight species in crude bovine bone extracts. The antibodies were used to immunohistochemically localize CIF-A/TGF-.beta. in fetal bovine bone and other tissues. There was abundant staining of osteocytes throughout cancellous and cortical bone as well as chondrocytes within the articular cartilage, although growth plate-associated chrondrocytes were not labeled. In addition, immunoreactive cells were detected in bone marrow (megakaryocytes and some mononuclear cells), fetal liver (hematopoietic stems cells), and the thymus (Hassall's corpuscle and some medullary thymocytes). In the kidney, the antibodies labeled a population of epithelial cells lining the calyces. Tissues which did not have detectable amounts of CIF-A/TGF-.beta. included the thyroid, adrenal, salivary gland, and aorta. Results presented here suggest that the factor may function in vivo as a general development and repair factor and may play a significant role in the differentiation of many cell types including chondrocytes, osteocytes, T-lymphocytes, and red blood cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004762908

Download citation: RISBibTeXText

Related references

Antibodies to the N-terminal portion of cartilage-inducing factor A and transforming growth factor beta. Immunohistochemical localization and association with differentiating cells. Journal of Biological Chemistry 261(26): 12362-12367, 1986

Antibodies to the N-terminal portion of cartilage-inducing factor A and transforming growth factor b. Immunohistochemical localization and association with differentiating cells. The Journal of Biological Chemistry 261: 362-7, 1986

Immunohistochemical localization of transforming growth factor-beta 1 in growth plate freshly transplanted into a full-thickness defect in articular cartilage. Nihon Seikeigeka Gakkai Zasshi 68(9): 790-797, 1994

Immunohistochemical localization of transforming growth factor tgf beta 1 in cardiac purkinje cells of the pig. Journal of Molecular and Cellular Cardiology 23(Suppl 5): S66, 1991

Immunohistochemical localization of transforming growth factor-alpha and transforming growth factor-beta during early human fetal prostate development. Journal of Urology 162(2): 509-513, 1999

Immunoelectron microscopic localization of transforming growth factor beta 1 and latent transforming growth factor beta 1 binding protein in human gastrointestinal carcinomas: qualitative difference between cancer cells and stromal cells. Cancer Research 53(1): 183-190, 1993

Immunohistochemical localization of basic fibroblast growth factor, platelet derived growth factor, transforming growth factor-beta and tumor necrosis factor-alpha in the pterygium. Acta Histochemica 98(2): 195-201, 1996

Growth stimulation of human breast cancer cells with anti-transforming growth factor beta antibodies: evidence for negative autocrine regulation by transforming growth factor beta. Cell Growth and Differentiation: the Molecular Biology Journal of the American Association for Cancer Research 1(8): 367-374, 1990

Cartilage-inducing factor-A. Apparent identity to transforming growth factor-beta. Journal of Biological Chemistry 261(13): 5693-5695, 1986

Repression of osteoblast and chondroblast phenotypes in vitro by transforming growth factor beta cartilage inducing factor a. Journal of Cell Biology 103(5 Part 2): 446A, 1986

Cartilage-inducing factor-B is a unique protein structurally and functionally related to transforming growth factor-beta. Journal of Biological Chemistry 262(5): 1946-1949, 1987

Immunohistochemical localization of epidermal growth factor, transforming growth factor-alpha and growth factor-beta s in the caprine peri-implantation period. Theriogenology 50(6): 931-944, 1998

Neutralizing antibodies against transforming growth factor beta potentiate the proliferation of Ki-1 positive lymphoma cells. Further evidence for negative autocrine regulation by transforming growth factor beta. American Journal of Pathology 140(3): 709-718, 1992

Immunohistochemical localization of transforming growth factor-beta 1, -beta 2, and -beta 3 latency-associated peptide in human cornea. IOVS Investigative Ophthalmology and Visual Science 35(8): 3289-3294, 1994

Modulation of transforming growth factor beta signalling pathway genes by transforming growth factor beta in human osteoarthritic chondrocytes: involvement of Sp1 in both early and late response cells to transforming growth factor beta. Arthritis Research and Therapy 13(1): R23, 2011