+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies



Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies



Journal of Infectious Diseases 151(4): 626-633



To study the antigenic characteristics of respiratory syncytial virus (RSV), monoclonal antibodies (MAbs) were developed to 3 strains of RSV and evaluated: 11 to Long, 4 to 18537, and 9 to A2. Of these, 6 MAbs immunoprecipitated the nucleoprotein, 6 the large glycoprotein and 11 the fusion protein. By the pattern of the reactions of these MAbs to 16 strains of RSV in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay (ELISA), 3 subgroups were distinguishable. With a panel of 10 of these 24 MAbs, 26 strains isolated from patients between 1979 and 1982 in Boston (Massachusetts, USA) were tested and 22 were found to belong to group 1, 4 to group 2 and none to group 3. The pattern of the reactions of the MAbs against representative strains from the 3 groups identified 9 epitopes by indirect immunofluorescence assay: 3 of each on the nucleoprotein, the large glycoprotein and the fusion protein. These results, along with those of previous studies, suggest that groups 1 and 3 are antigenically similar and group 2 is antigenically more distinct.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004765890

Download citation: RISBibTeXText

PMID: 2579169

DOI: 10.1093/infdis/151.4.626


Related references

Preparation and characterization of monoclonal antibodies directed against human respiratory syncytial virus subgroup b strain and antigenic analysis of subgroup b field strains with these monoclonal antibodies. Sapporo Medical Journal 59(3): 215-226, 1990

Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. Journal of General Virology 73: 2225-2234, 1992

Monoclonal antibodies to respiratory syncytial virus and their application in identify the virus protein specificity and comparing their antigenic difference between virus isolates. Virologica Sinica 7(2): 181-185, 1992

Monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. Journal of General Virology 68: 2161-2167, 1987

Heterogeneity of respiratory syncytial virus rsv strains demonstrated by differential evolution of epitopes using monoclonal antibodies to strains of epidemiologically distinct outbreaks. Pediatric Research 23(4 Part 2): 383A, 1988

The characterization and uses of monoclonal antibodies to respiratory syncytial virus. Developments in Biological Standardization 57: 237-244, 1984

Analysis of the binding of monoclonal and polyclonal antibodies to the glycoproteins of antigenic variants of human respiratory syncytial virus by surface plasmon resonance. Journal of Immunological Methods 297(1-2): 143-152, 2005

Production and characterization of bovine monoclonal antibodies to respiratory syncytial virus. Journal of General Virology 69: 3023-3032, 1988

Production and characterization of monoclonal antibodies to bovine respiratory syncytial virus. Veterinary Microbiology 43(2-3): 261-274, 1995

Monoclonal antibodies demonstrate heterogeneity in the G glycoprotein of prototype strains and clinical isolates of respiratory syncytial virus. Journal of Medical Virology 22(4): 345-356, 1987

Respiratory syncytial virus immunoglobulin and monoclonal antibodies in the prevention and treatment of respiratory syncytial virus infection. Seminars in Perinatology 22(1): 87-95, 1998

Antigenic and genetic characterization of twenty-six strains of human respiratory syncytial virus (subgroup A) isolated during three consecutive outbreaks in Havana city, Cuba. Memorias do Instituto Oswaldo Cruz 94(4): 469-475, 1999

Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B. Journal of General Virology 68: 3125-3135, 1987

Molecular characterization of an outbreak of respiratory syncytial virus in Havana, Cuba, by monoclonal antibodies and restriction mapping. Clinical & Diagnostic Laboratory Immunology 4(5): 633-634, 1997

Antigenic characterization of porcine respiratory coronavirus with the aid of monoclonal antibodies against transmissible gastroenteritis virus. Journees de la recherche porcine en France: 0th) 89-93, 1988