+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins

Antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins

Journal of Virology 61(8): 2607-2613

The antigenic relationships among seven feline coronavirus isolates were investigated by using a panel of 26 monoclonal antibodies (MAbs). The MAbs were categorized into five immunoreactive groups which were used to delineate two antigenic types of feline coronaviruses. One antigenic type included the more virulent feline infectious peritonitis virus (FIPV) isolates (FIPV-UCD-1, FIPV-UCD-4, FIPV-TN406, FIPV-DF2, and FIPV-79-1146), whereas the second antigenic type was composed of the avirulent isolate FIPV-UCD-2. The feline enteric coronavirus isolate FECV-79-1683 shared some characteristics of both of the major antigenic groups. Epitopes on the nucleocapsid and envelope polypeptides were in general highly conserved among both antigenic types, although a few type-specific antigenic sites were discriminated. The most striking finding was the marked antigenic difference in the peplomer (E2) glycoproteins between the two antigenic types. Seven anti-E2 MAbs reacted with one antigenic type of E2, whereas seven other anti-E2 MAbs recognized a different antigenic form of E2. None of the 14 anti-E2 MAbs reacted with all of the isolates.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 004765910

Download citation: RISBibTeXText

PMID: 3599183

Related references

Functional differences in the peplomer glycoproteins of feline coronavirus isolates. Journal of Virology 61(8): 2655-2657, 1987

Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE. Journal of General Virology 73: 1725-1737, 1992

Antigenic and biological comparison of feline coronavirus isolates. Dissertation Abstracts International, B 47(11): 4410, 1987

Enteric coronavirus TGEV: mapping of four major antigenic determinants in the amino half of peplomer protein E 2. Advances in Experimental Medicine and Biology 276: 151-157, 1990

The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (TGEV): comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (FIPV). Virus Research 8(4): 363-371, 1987

Detection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis. Veterinary Pathology 45(2): 140-153, 2008

The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Research 5(2-3): 253-263, 1986

Plaque assay, polypeptide composition and immunochemistry of feline infectious peritonitis virus and feline enteric coronavirus isolates. Advances in Experimental Medicine and Biology 173: 133-147, 1984

Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genes. Virus Research 13(2): 87-100, 1989

The peplomer protein sequence of the m 41 strain of coronavirus infectious bronchitis virus and its comparison with beaudette strains. Virus Research 5(2-3): 253-264, 1986

Feline infectious peritonitis: role of the feline coronavirus 3c gene in intestinal tropism and pathogenicity based upon isolates from resident and adopted shelter cats. Virus Research 165(1): 17-28, 2012

Antigenic variation among murine coronaviruses: evidence for polymorphism on the peplomer glycoprotein, E2. Virus Research 2(4): 317-328, 1985

Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. Journal of Virology 72(5): 4508-4514, 1998

Feline immunodeficiency virus in Switzerland: clinical and epidemiological features in comparison with feline leukaemia virus and feline coronavirus. Schweizer Archiv fur Tierheilkunde 132(5): 217-225, 1990

The feline coronavirus infections: feline infectious peritonitis and feline coronavirus enteritis. Veterinary Medicine 86(4): 376...393, 1991