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API ZYM and API An-Ident reactions of fastidious oral gram-negative species


API ZYM and API An-Ident reactions of fastidious oral gram-negative species



Journal of Clinical Microbiology 22(3): 333-335



ISSN/ISBN: 0095-1137

PMID: 3930558

API ZYM and API An-Ident enzymatic substrate tests were done on 6 oral species which are difficult to characterize with conventional biochemical tests. Bacteroides forsythus, the fusiform Bacteroides spp., is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. The tests were able to separate this new species from species of Capnocytophaga and Fusobacterium. B. forsythus reactions were similar but not identical to those of reference Bacteroides spp. Positive reactions for .alpha.-glucosidase, .beta.-glucosidase, .alpha.-fucosidase, and .alpha.-glucuronidase suggest that B. forsythus may be saccharolytic. It was the only species tested which was trypsin positive. Wolinella sp., Campylobacter coniscus, B. gracilis and Eikenella corrodens are asaccharolytic, and characterization relies heavily on sensitivities to inhibitory agents. These species reacted weakly in the API ZYM and API An-Indent enzymatic substrate tests, and the reactions were not useful for separating these species. The enzyme reactions differentiated W. recta and C. coniscus from Selenomonas sputigena, another oral motile but saccharolytic organism.

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Accession: 004770147

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