+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Assignment of thermoluminescence a band to s 3 negative charge on the primary quinone acceptor of photosystem ii charge recombination sequential stabilization of s 3 and negative charge on the primary quinone acceptor of photosystem ii by a two step illumination at different temperatures



Assignment of thermoluminescence a band to s 3 negative charge on the primary quinone acceptor of photosystem ii charge recombination sequential stabilization of s 3 and negative charge on the primary quinone acceptor of photosystem ii by a two step illumination at different temperatures



Biochimica et Biophysica Acta 850(1): 80-89



The origin of thermoluminescence A band emitted from Photosystem II was studied by analyzing the dependence of the charging efficiency of the A band by continuous illumination at - 196.degree. C on preillumination flashes and DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea], treatment. The following results were obtained. (1) Illumination at - 196.degree. C given after preillumination flashes at 15.degree. C altered the normal oscillation pattern of the flash-induced B band in dark-adapted [spinach] thylakoids having emission maxima at the first and fifth flashes to a very different pattern showing huge maxima at the second and sixth flashes. (2) The charging efficiency of the A band by the illumination at - 196.degree. C oscillated in parallel with the altered oscillation pattern of the B band. (3) Addition of DCMU after preillumination flashes but prior to illumination at - 196.degree. C strongly enhanced the A band at the expense of the B band, and the oscillation pattern of the A band thus obtained was almost identical to that of the B band observed in the absence of DCMU. (4) These phenomena were satisfactorily explained by a sequential formation of specified positive and negative charges at the reaction center by flash preillumination and continuous illumination at - 196.degree. C, respectively. (5) In CaCl2-washed Photosystem II particles, where the S3-S4 transition is blocked by removal of the three extrinsic proteins, the A-band height as a function of the flash number increased up to second flash but stopped oscillating thereafter. Based on these results, it was concluded that the A band of thermoluminescence arises from recombination between the negative charge on the primary quinone acceptor of Photosystem II .**GRAPHIC**. and the positive charge on the S3 state of the O2-evolution system.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004789296

Download citation: RISBibTeXText


Related references

Assignment of thermoluminescence A band to S3QA-charge recombination: sequential stabilization of S3 and QA- by a two-step illumination at different temperatures. Biochimica et biophysica acta0, 850(1): 80-89, 1986

Charge accumulation and recombination in photosystem II studied by thermoluminescence. I. Participation of the primary acceptor Q and secondary acceptor B in the generation of thermoluminescence of chloroplasts. Biochimica et biophysica acta: bioenergetics 764(1): 24-32, 1984

Charge separation is virtually irreversible in photosystem II core complexes with oxidized primary quinone acceptor. Journal of Physical Chemistry. a 115(16): 3947-3956, 2011

Charge accumulation and recombination in photosystem ii studies by thermo luminescence 1. participation of the primary acceptor q and secondary acceptor b in the generation of thermo luminescence of chloroplasts. Biochimica et Biophysica Acta 764(1): 24-32, 1984

Oxygen-evolving Photosystem II core complexes: a new paradigm based on the spectral identification of the charge-separating state, the primary acceptor and assignment of low-temperature fluorescence. Photochemical & Photobiological Sciences 4(9): 744-753, 2005

Evidence from thermoluminescence for bicarbonate action on the recombination reactions involving the secondary quinone electron acceptor of photosystem ii. Biochimica et Biophysica Acta 766(2): 416-423, 1984

Thylakoid photosystem ii activity supported by the non quinone acceptor q 400 and an ancillary quinone acceptor aq. Biochimica et Biophysica Acta 935(2): 225-235, 1988

Thermoluminescence study of charge recombination in photosystem ii at low temperatures i. characterization of the z v and a thermoluminescence bands. Biochimica et Biophysica Acta 809(3): 369-378, 1985

The primary quinone electron acceptor of photosystem II may be the photosensitizer for UV-B damage. Plant Physiology & Biochemistry 38: s175, 2000

Silicomolybdate substitutes for the function of a primary electron acceptor and stabilizes charge separation in the photosystem ii reaction center complex. Febs Letters 255(1): 133-138, 1989

Involvement of bicarbonate in the protonation of the secondary quinone electron acceptor of photosystem ii via the non heme iron of the quinone iron acceptor complex. Febs Letters 226(2): 347-351, 1988

Thermoluminescence study of charge recombination in Photosystem II at low temperatures. II. Oscillatory properties of the Zv and A thermoluminescence bands in chloroplasts dark-adapted for various time periods. Biochimica et Biophysica Acta - Bioenergetics 809(3): 379-387, 1985

Thermoluminescence study of charge recombination in photosystem ii at low temperatures ii. oscillatory properties of the z v and a thermoluminescence bands in chloroplasts dark adapted for various time periods. Biochimica et Biophysica Acta 809(3): 379-387, 1985

Functional reconstitution of the primary quinone acceptor, Q-A, in the photosystem II core complexes. Biochimica Et Biophysica Acta. 1142(1): 36-42, 1993

Characterization by FTIR spectroscopy of the photoreduction of the primary quinone acceptor QA in photosystem II. FEBS Letters 269(2): 363-367, 1990