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Atp adp and amp content of spermatozoa of domestic animals and man and of bull spermatozoa during deep freeze preservation



Atp adp and amp content of spermatozoa of domestic animals and man and of bull spermatozoa during deep freeze preservation



Zuchthygiene (Berlin) 17(2): 49-55



The content of ATP, ADP and AMP in sperm of men, bulls, stallions, rams, goats, rabbits, boars, dogs and cocks was investigated by means of the bioluminescent measurement. The content of ATP was higher than ADP or AMP in all species except the cock. The adenylate energy charge was between 0.6 and 0.75. The ATP level was correlated with the usual criteria, motility estimation and supravital staining for sperm stored at +38.degree. C for 18 h. During the deepfreezing of bull semen, the unfractioned addition of glycerol or cooling were more clear determinated by a fall in ATP level than a reduction in motility. The 3 adenosine nucleotides, ATP, ADP and AMP reacted differnetly during the freezing procedure; the ATP level fell, the ADP level remained nearly constant, and the AMP level increased in the course of deepfreezing and fell after thawing.

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Accession: 004793930

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