Attempts to propagate the scrapie agent in vitro in mouse neural cells
Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R.
Comptes Rendus des Seances de l'Academie des Sciences Serie III Sciences de la Vie 293(8): 413-418
ISSN/ISBN: 0249-6313 Accession: 004796278
Attempts were made to propagate the serapie agent in vitro in mouse glia and neuroblastoma cells. After several passages in vitro, yielding at most 10-9 final dilution of the original material, the extracts of 4 of 7 cultures, when injected i.c. into CD1 mice, produced a deadly disease displaying the clinical and pathological signs characteristic of serapie.