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Auto immune interstitial disease of the kidney and associated antigen purification and characterization of a soluble tubular basement membrane antigen



Auto immune interstitial disease of the kidney and associated antigen purification and characterization of a soluble tubular basement membrane antigen



Clinical Immunology & Immunopathology 19(3): 360-371



A soluble tubular basement membrane (TBM) antigen was purified from human kidneys by a combination of immunochemical and physicochemical procedures. Its chemical properties were entirely different from those of glomerular basement membrane (GBM) antigen as studied by DEAE-cellulose chromatography and electrophoresis. The MW of TBM antigen was estimated to be 30,000 daltons and that of GBM 40,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified TBM antigen did not cross-react with GBM antigen and cross-reacted with the TBM antigen similarly purified from other animals such as goats, guinea pigs and mice in immunodiffusion plates. Goats and some mice immunized with the purified human TBM antigen developed a typical interstitial nephritis. The goat sera stained the basement membrane of normal tubules and Bowmann's capsules, and no anti-GBM activity was detected in the serum by immunofluorescence. The system provides a simple and useful interstitial nephritis model produced with a purified TBM antigen.

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Accession: 004799820

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PMID: 6788418



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