Automated assay for alpha 1-antitrypsin with N-alpha-benzoyl-DL-arginine-p-nitroanilide as trypsin substrate and standardized with p-nitrophenyl-p'-guanidinobenzoate as titrant for trypsin active sites

Eckfeldt, J.H.; Light, R.T.; Leiendecker-Foster, C.

Clinical Chemistry 28(5): 1108-1112

1982


ISSN/ISBN: 0009-9147
PMID: 6280895
Accession: 004801473

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
.alpha.1-Antitrypsin is the most abundant of several serum protease inhibitors. Its deficiency is associated with an increased incidence of emphysema in adults, jaundice in newborns and childhood cirrhosis. An automated functional assay for the Instrumentation Laboratory's Multistat III Microcentrifugal Analyzer is described with N-.alpha.-benzoyl-DL-arginine-p-nitroanilide as trypsin substrate. The assay is standardized in terms of moles of trypsin active sites inhibited per liter of serum, by use of chromogenic titrant for trypsin active sites, p-nitrophenyl-p'-guanidinobenzoate. The method is rapid, precise and independent of trypsin supplier, and results correlate well with those by a manual chromogenic and a nephelometric assay.