+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Automated methods for estimation of sperm flagellar bending parameters

Automated methods for estimation of sperm flagellar bending parameters

Cell Motility 4(6): 417-430

Parameters to describe flagellar bending patterns can be obtained by a microcomputer procedure that uses a set of parameters to synthesize model bending patterns, compares the model bending patterns with digitized and filtered data from flagellar photographs, and uses the Simplex method to vary the parameters until a solution with minimum root mean square differences between the model and the data is found. Parameters for Chlamydomonas bending patterns have been obtained from comparison of shear angle curves for the model and the data. To avoid the determination of the orientation of the basal end of the flagellum, which is required for calculation of shear angles, parameters for sperm flagella have been obtained by comparison of curves of curvature as a function of length for the model and for the data. A constant curvature model, modified from that originally used for Chlamydomonas flagella, was used for obtaining parameters from sperm flagella, but the methods can be applied using other models for synthesizing the model bending patterns.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004801861

Download citation: RISBibTeXText

PMID: 6542452

DOI: 10.1002/cm.970040603

Related references

An automated method for measuring the flagellar bending parameters. Biorheology 26(3): 532, 1989

Sperm-activating peptide induces asymmetric flagellar bending in sea urchin sperm. Zoological Science 22(3): 293-299, 2005

Analysis of the flagellar bending waves of ejaculated ram sperm. Cell Motility and the Cytoskeleton 8(3): 261-273, 1987

Regulation of flagellar bending by cAMP and Ca2+ in hamster sperm. Molecular Reproduction and Development 53(1): 77-83, 1999

Modulation of the asymmetry of sea urchin sperm flagellar bending by calmodulin. Journal of Cell Biology 100(6): 1875-1883, 1985

Initiation of hyperactivated flagellar bending in mouse sperm within the female reproductive tract. Biology of Reproduction 36(5): 1191-1198, 1987

A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm. Reproduction 144(1): 101-109, 2012

The effect of flagellar bending of asterosaps, sperm-activating peptides of the starfish, Asterias amurensis. Zoological Science 19(12): 1434, 2002

Studies on generation and propagation of sperm flagellar bending wave by means of fluorescent probe. Zoological Science 18: 35, 2001

Hyperactivated flagellar bending is initiated in mouse sperm prior to ovulation and isthmic motility suppression. Biology of Reproduction 34(Suppl. 1): 190, 1986

Mechanism of flagellar oscillation-bending-induced switching of dynein activity in elastase-treated axonemes of sea urchin sperm. Journal of Cell Science 121(Pt 17): 2833-2843, 2008

Control of flagellar bending parameters the roles of cyclic amp dependent phosphorylation and calmodulin. Cell Motility: 163-164, 1985

Description and performance of a computer automated sperm flagellar analysis system. Journal of Cell Biology 105(4 Part 2): 34A, 1987

Automated analysis of rabbit sperm motility and the effect of chemicals on sperm motion parameters. Molecular Reproduction and Development 33(3): 347-356, 1992

Compartmentalization of a unique ADP/ATP carrier protein SFEC (Sperm Flagellar Energy Carrier, AAC4) with glycolytic enzymes in the fibrous sheath of the human sperm flagellar principal piece. Developmental Biology 302(2): 463-476, 2007