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Binding of murine immuno globulin m and mono clonal immuno globulin m a k hybrid anti immuno globulin type iii pneumococcal poly saccharide antibodies to staphylococcal protein a



Binding of murine immuno globulin m and mono clonal immuno globulin m a k hybrid anti immuno globulin type iii pneumococcal poly saccharide antibodies to staphylococcal protein a



Australian Journal of Experimental Biology & Medical Science 61(1): 91-104



Evidence is produced to show that 2 specific subclasses of IgM antibodies are formed during the primary immune response to Type III pneumococcal polysaccharide (SIII) in mice. The IgM proteins can be divided into 2 groups based on different reactivities with protein-A. As with human IgM subclasses, it is proposed to call the 2 IgM subclasses IgM1 and IgM2, where the latter is defined by the ability to react with protein-A of Staphylococcus aureus. Only the IgM molecule which reacted with protein-A produced passive hemolysis in the presence of guinea-pig complement. Results also show that the IgA anti-SIII activity in serum of SIII-immune mice on day 5 of the primary repsonse is due to hybrid IgM/A (.kappa.) antibody which is undetected by conventional methods for enumerating antibody-forming cells because cells producing IgM/A antibody develop direct plaques with guinea-pig complement. A monoclonal IgM/A(.kappa.) anti-SIII produced from spleen cells 5 days after injection of 10 .mu.g SIII plus pertussis vaccine exhibited similar properties to molecules with .mu. and .alpha.-determinants in serum. The IgM/A hybrid antibody reacted strongly with protein-A and produced passive hemolysis of SIII-coated erythrocytes in the presence of guinea-pig, but not mouse, complement. Despite its specificity for the capsular antigen of Type III pneumococci, the IgM/A hybrid antibody conferred only temporary immunity in mice challenged with viable pneumococci. The nature and properties of IgM/A antibodies as well as those of the subclasses of IgM may give important clues to the genetic regulation and expression of antibody production. These findings may provide an explanation for some of the anomalies in various areas of immunological research.

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