Biochemical adaptive responses in glucose metabolism of fish tilapia mossambica during ammonia toxicity

Begum, S.J.

Current Science 56(14): 705-708

1987


ISSN/ISBN: 0011-3891
Accession: 004830626

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Abstract
Changes in glucose metabolism were studied in the liver and gill of freshwater teleost. Tilapia mossambica exposed to a sub-lethal concentration (0.001 M) of ammonia. A decrease in glycogen content and an increase in phosphorlyase and aldolase activity levels suggested rapid mobilization of glycogen through glycolytic pathway. Changes in lactate, pyruvate and lactate dehydrogenase were found to be tissue-specific. Glucose-6-phosphate dehydrogenase activity was increased throughout the time course study suggested enhanced oxidation of glucose by the HMP shunt. The activity levels of citric acid cycle enzymes such as isocitrate, succinate and malate dehydrogenases and cytochrome-C-oxidase were found to be altered as a function of time during ammonia impact. A slight elevation in AMP levels with a significant drop in ATP, ADP and energy charge was observed. The levels of ATP and ADP showed a rapid decrement during the early phase of ammonia stress probably due to their increased utilization than at the later phase of exposure. The metabolic changes induced by ambient ammonia in fish are found to be tissue-specific as well as time-dependent.